Regulatory

Part:BBa_K233005:Design

Designed by: swetha srinivasan, samit watve, mandar phatak, chinar patil   Group: iGEM09_IBB_Pune   (2009-07-10)

AND gate 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We obtained a lac operator and paired it with phage pLL promoter. Hence we have an AND gate which requires both the inputs- phage encoded protein Ogr and IPTG/lactose in order for the gene downstream to be transcribed.

Source

E.coli- lac operator pLL- phage p4

References